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Human papillomavirus 16 detected in nasopharyngeal carcinomas in white Americans but not in endemic Southern Chinese patients
Author(s) -
Lin Zhixiong,
Khong Brian,
Kwok Shirley,
Cao Hongbin,
West Robert B.,
Le QuynhThu,
Kong Christina S.
Publication year - 2014
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23362
Subject(s) - nasopharyngeal carcinoma , cohort , medicine , human papillomavirus , oncology , pathology , radiation therapy
Background We evaluated the relationship of human papillomavirus (HPV) and Epstein–Barr virus (EBV) with race in endemic and nonendemic cohorts of patients with nasopharyngeal carcinoma (NPC), and with smoking status in the nonendemic cohort. Methods Tissue microarrays (TMAs) were constructed using samples from 86 patients treated in southern China and 108 patients from Stanford, California. TMAs were stained with p16, HPV in situ hybridization (ISH), and EBV ISH. Polymerase chain reaction (PCR) was used to confirm EBV(‐) cases and HPV status in p16(+) cases. Survival data was available for the Stanford cohort only. Results No HPV(+) cases were detected in the Chinese cohort. In the Stanford cohort, 5 of 11 EBV(‐) cases harbored HPV‐16, 10 of 10 occurred in whites, and 8 of 11 were smokers. Patients with EBV(‐) NPC also showed a trend toward worse survival. Conclusion EBV(‐) NPC shows an association with the presence of HPV, white race, and smoking. In contrast, EBV(‐) NPC shows no association with HPV in the endemic cohort. © 2013 Wiley Periodicals, Inc. Head Neck 36 : 709–714, 2014