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Telomerase‐specific oncolytic adenovirus: Antitumor effects on radiation‐resistant head and neck squamous cell carcinoma cells
Author(s) -
Takahashi Hideaki,
Hyakusoku Hiroshi,
Horii Chihiro,
Takahashi Masahiro,
Nishimura Goshi,
Taguchi Takahide,
Kondo Norio,
Sakakibara Atsuko,
Urata Yasuo,
Sano Daisuke
Publication year - 2014
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23309
Subject(s) - radioresistance , head and neck squamous cell carcinoma , cancer research , oncolytic virus , radiation therapy , apoptosis , telomerase , oncolytic adenovirus , in vivo , radiosensitivity , medicine , cell , dna repair , oncology , biology , head and neck cancer , dna , tumor cells , biochemistry , genetics , microbiology and biotechnology , gene
Background Radioresistance remains a critical issue in the use of radiotherapy for the treatment of head and neck squamous cell carcinoma (HNSCC). This study evaluated the efficacy of combination treatment with OBP‐301, a telomerase‐specific replication‐selective adenovirus, and radiotherapy in overcoming radioresistance by examining its effect on radiation‐resistant HNSCC cells. Methods Radiation‐resistant HNSCC cells were treated with OBP‐301 and radiation in vitro and in an orthotopic nude mouse model in vivo and synergism was assessed. Apoptosis and expression of MRN complex, which plays a key role in DNA repair machinery, were also analyzed. Results Infection with OBP‐301 was found to enhance the antitumor efficacy of radiation both in vitro and in vivo by inhibiting MRN complex expression and increasing apoptosis induction. Conclusion Combined OBP‐301 and radiation therapy seems to overcome radioresistance in HNSCC cells by inhibiting DNA repair machinery, and may thus be a novel therapeutic strategy for treating HNSCC. © 2013 Wiley Periodicals, Inc. Head Neck 36 : 411–418, 2014