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New genetic findings in parotid gland pleomorphic adenomas
Author(s) -
Wemmert Silke,
Willnecker Vivienne,
Brunner Christian,
Wenzel Gentiana Ioana,
Sauter Birgit,
Meinelt Heike,
Bartholmé Nadia,
Saada Carolin,
Bohle Rainer Maria,
Urbschat Steffi,
Schick Bernhard
Publication year - 2013
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23147
Subject(s) - pleomorphic adenoma , comparative genomic hybridization , biology , fluorescence in situ hybridization , salivary gland , parotid gland , chromosome , pathology , carcinoma ex pleomorphic adenoma , cytogenetics , carcinogenesis , myoepithelial cell , gene , genetics , medicine , immunohistochemistry
Background Despite numerous studies, the tumor biology of pleomorphic adenomas, the most common salivary gland tumors, is still not completely defined. In order to identify further candidate genes important for tumor biology of pleomorphic adenomas, extended cytogenetic and molecular analysis are mandatory. Methods We performed a detailed molecular cytogenetic analysis using comparative genomic hybridization (CGH) followed by fluorescence in situ hybridization (FISH) with probes for chromosome X, 16p, 17, and 20 on a large cohort of pleomorphic adenomas ( n = 29). Results We could confirm previously described deletions in pleomorphic adenomas affecting 16p, 17, 20q, and 22 by FISH and/or CGH analysis. Moreover, our CGH study revealed novel candidate regions on 8p23.1pter, 9p, 10q25.1q25.3, and 11q24qter in the series of analyzed pleomorphic adenomas. Conclusion Our present study reveals new insights in novel candidate regions implicated in pleomorphic adenoma tumorigenesis which should be considered in further molecular studies. © 2012 Wiley Periodicals, Inc. Head Neck 35: 1431–1438, 2013