Premium
Viral load, physical status, and E6/E7 mRNA expression of human papillomavirus in head and neck squamous cell carcinoma
Author(s) -
Deng Zeyi,
Hasegawa Masahiro,
Kiyuna Asanori,
Matayoshi Sen,
Uehara Takayuki,
Agena Shinya,
Yamashita Yukashi,
Ogawa Kazuhiko,
Maeda Hiroyuki,
Suzuki Mikio
Publication year - 2013
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23034
Subject(s) - head and neck squamous cell carcinoma , viral load , human papillomavirus , messenger rna , cancer research , polymerase chain reaction , cell , real time polymerase chain reaction , oncology , head and neck cancer , medicine , virus , biology , cancer , virology , gene , biochemistry , genetics
Background The purpose of this study was to determine prospectively both human papillomavirus (HPV) load and physical status in different types of head and neck squamous cell carcinoma (HNSCC). Methods HPV DNA, E6/E7 mRNA expression, viral load, and physical status of 184 patients with HNSCC were examined simultaneously by polymerase chain reaction (PCR)‐based methods. Results The HPV genome was detected in 54 HNSCC samples (29.3%), particularly in tonsillar carcinomas (69.6%). Compared with nonoropharyngeal HNSCC, oropharyngeal carcinoma harbored a relatively higher viral load, especially in tonsillar carcinoma. Although integrated or mixed status was observed in 75.6% of HPV‐16–positive samples, E6/E7 mRNA transcripts were detected in only 27.5% of HPV DNA‐positive cases. High HPV‐16 load correlated significantly with E6/E7 mRNA expression. Conclusion E6/E7 mRNA expression in patients with HNSCC with low viral load remains low even in cases of integration to the host genome. Tonsillar carcinomas were significantly associated with HPV among various types of HNSCC. © 2012 Wiley Periodicals, Inc. Head Neck, 2012