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Suppression of natural killer‐cell and dendritic‐cell apoptotic tumoricidal activity in patients with head and neck cancer
Author(s) -
Baskic Dejan,
Vujanovic Lazar,
Arsenijevic Nebojsa,
Whiteside Theresa L.,
Myers Eugene N.,
Vujanovic Nikola L.
Publication year - 2013
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.22968
Subject(s) - apoptosis , natural killer cell , cell , head and neck cancer , cancer , head and neck , dendritic cell , medicine , cancer research , immunology , cytotoxicity , biology , immune system , in vitro , surgery , biochemistry , genetics
Background Natural killer (NK) cells and dendritic cells (DCs) mediate tumor cell apoptosis using tumor necrosis factor superfamily ligands (TNFSFLs). This cytotoxicity is an important anticancer immune defense mechanism. Methods We examined TNFSFL expression and apoptotic tumoricidal activity (ATA) of purified NK cells and DCs, and peripheral blood mononuclear leukocytes (PBMLs) of healthy individuals and patients with head and neck cancer (HNC) before and after cancer ablation. Results PBMLs, NK cells and DCs, but not NK‐cell/DC‐depleted PBMLs, expressed multiple TNFSFLs and mediated ATA. Both TNFSFL expression and ATA were suppressed in tumor‐bearing, and restored in tumor‐ablated patients with (HNC) Soluble TNF superfamily receptors (solTNFSFRs) were increasingly bound by PBNLs of tumor‐bearing HNC patients. Dissociation of solTNFSFR led to more pronounced increases in TNFSFL expression and ATA of PBMLs of patients with HNC than healthy individuals. Conclusion NK‐cell and DC TNFSFL expression and ATA are suppressed in patients with HNC. This suppression is tumor‐dependent and possibly mediated by solTNFSFRs. © 2012 Wiley Periodicals, Inc. Head Neck, 2013