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Lysyl oxidase and enhancement of cell proliferation and angiogenesis in oral squamous cell carcinoma
Author(s) -
Shih YinHua,
Chang KuoWei,
Chen Michael Yuanchien,
Yu ChengChia,
Lin DanJae,
Hsia ShihMin,
Huang HengLi,
Shieh TzongMing
Publication year - 2013
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.22959
Subject(s) - lysyl oxidase , angiogenesis , cell growth , carcinogenesis , elastin , cancer research , chemistry , cd31 , extracellular matrix , cell culture , transfection , microbiology and biotechnology , biology , biochemistry , genetics , gene
Background Lysyl oxidase (LOX) is a copper‐dependent enzyme that cross‐links collagen and elastin in the extracellular matrix. LOX overexpressed in various tumors. The manner in which LOX affects tumor growth remains controversial. Methods Chemical treatment and gene transfection were used to induce LOX overexpression or inhibition in cell lines SAS and SVEC4‐10. LOX mRNA , protein, and activity were confirmed before tube formation assay and tumorigenesis. The microvessels in the tumor section were detected by immunostaining CD31‐positive endothelial cells. Results LOX overexpression and copper induction of LOX activity increased SVEC4‐10 tube formation. LOX silencing and β‐aminopropionitrile inhibition of LOX activity had opposite effects. LOX overexpression increased proliferation and proliferating cell nuclear antigen expression. High LOX expression clones increased tumor size in a tumorigenesis model. The microvascular numbers were higher in LOX overexpression tumors than in control tumors. Conclusion LOX can induce cell proliferation and angiogenesis in oral squamous cell carcinoma. © 2012 Wiley Periodicals, Inc. Head Neck, 2013