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Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP‐2 induced by an adeno‐associated virus expressing human FAS ligand
Author(s) -
Sun Haili,
Liu Yuhe,
Bu Dingfang,
Liu Xiang,
Norris James S.,
Xiao Shuifang
Publication year - 2012
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21985
Subject(s) - fas ligand , apoptosis , adeno associated virus , flow cytometry , genetic enhancement , cancer research , green fluorescent protein , cell culture , head and neck squamous cell carcinoma , microbiology and biotechnology , virus , nude mouse , biology , virology , gene , programmed cell death , cancer , head and neck cancer , biochemistry , genetics , vector (molecular biology) , recombinant dna
Abstract Background Apoptosis induced by Fas/FasL system has been proposed as a gene therapy methold for various cancers. Methods We used adeno‐associated virus–expressing enhanced green fluorescent protein (EGFP)‐human FasL (AAV‐EGFP‐hFasL) to deliver FasL into Hep‐2 cells, cytotoxicity was detected by MTS assay , apoptosis was confirmed by flow cytometry. We also treated the xenograft of Hep‐2 tumor in nude mice with intratumoral injection of AAV‐EGFP‐hFasL. The size of the xenograft, the apoptosis in the xenograft, and the survival rate of the inoculated mice were then evaluated. Results Hep‐2 cells infected with AAV‐EGFP‐hFasL showed increased apoptosis rate and killing effect compared with AAV‐EGFP–infected cells. In addition intratumoral injections of AAV‐EGFP‐hFasL into Hep‐2 xenografts induced significant growth suppression of tumors. Conclusion Our findings suggest that the introduction of FasL into head and neck squamous cell carcinoma may induce significant apoptosis, and adeno‐associated virus may be a useful vehicle for gene therapy. © 2012 Wiley Periodicals, Inc. Head Neck , 2012