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Tissue imprint for molecular mapping of deep surgical margins in patients with head and neck squamous cell carcinoma
Author(s) -
Roh Jong–Lyel,
Westra William H.,
Califano Joseph A.,
Sidransky David,
Koch Wayne M.
Publication year - 2012
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21982
Subject(s) - head and neck squamous cell carcinoma , dna methylation , methylation , pathology , medicine , head and neck , head and neck cancer , cancer , cancer research , biology , dna , gene , surgery , gene expression , biochemistry , genetics
Background Tissue imprinting can generate molecular marker maps of tumor cells at deep surgical margins. The purpose of this study was to evaluate the feasibility of this method for detection of residual head and neck squamous cell carcinoma (HNSCC). Methods Paired fresh tissue and nitrocellulose membrane imprints of tumor and deep margins were collected from 17 HNSCC resections. DNA was amplified using quantitative methylation‐specific polymerase chain reaction (qMSP) for p16 , DCC , KIF1A , and EDNRB . Levels of methylation in tumors and deep margins were compared. Results DNA from imprints was adequate for qMSP. Hypermethylation of target genes was present in 12 of 17 tumors and in 8 deep margins. Methylation level was better from margin imprints than tissue. During follow‐up (median, 13 months), local or regional recurrences occurred in 6 cases of which 5 had molecularly positive margins. Conclusion Tissue imprinting is feasible for molecular detection of residual tumor at deep surgical margins and may correlate with locoregional recurrence. © 2012 Wiley Periodicals, Inc. Head Neck , 2012