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UM‐SCC‐104: A New human papillomavirus‐16–positive cancer stem cell–containing head and neck squamous cell carcinoma cell line
Author(s) -
Tang Alice L.,
Hauff Samantha J.,
Owen John H.,
Graham Martin P.,
Czerwinski Michael J.,
Park Jung Je,
Walline Heather,
Papagerakis Silvana,
Stoerker Jay,
McHugh Jonathan B.,
Chepeha Douglas B.,
Bradford Carol R.,
Carey Thomas E.,
Prince Mark E.
Publication year - 2012
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21962
Subject(s) - aldehyde dehydrogenase , head and neck squamous cell carcinoma , cancer stem cell , cancer research , cell , cell culture , pathology , head and neck cancer , cancer , stem cell , medicine , biology , gene , biochemistry , genetics
Background Few human papillomavirus (HPV)(+) head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established University of Michigan‐squamous cell carcinoma‐104 (UM‐SCC‐104), a new HPV(+) HNSCC cell line from a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSCs). Methods Tumor cells were tested for biomarker expression by immunohistology, and the presence of HPV was assessed by several methods. Results UM‐SCC‐104 has a unique genotype, contains HPV‐16, and expresses E6/E7. Inoculation of aldehyde dehydrogenase (ALDH)(+) and ALDH(−) cells in an immunocompromised mouse resulted in tumor growth from the ALDH(+) cells after 6 weeks that recapitulated the histology of the primary, whereas ALDH(−) cells did not produce tumors. Conclusion UM‐SCC‐104, a new HPV‐16, CSC‐containing HNSCC cell line will aid in studying recurrent HPV(+) tumors. The aggressive nature of this tumor is consistent with high uniform expression of epidermal growth factor receptor (EGFR) and a functionally significant proportion of ALDH(+) CSCs. © 2011 Wiley Periodicals, Inc. Head Neck , 2011