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Role of insulin‐like growth factor‐II mRNA‐binding protein‐3 in invadopodia formation and the growth of oral squamous cell carcinoma in athymic nude mice
Author(s) -
Hwang Young Sun,
Park KwangKyun,
Cha In Ho,
Kim Jin,
Chung WonYoon
Publication year - 2012
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21929
Subject(s) - invadopodia , extracellular matrix , cancer research , microbiology and biotechnology , cortactin , cancer cell , gene silencing , cancer , biology , chemistry , cell , biochemistry , cytoskeleton , genetics , gene
Abstract Background The invadopodia are specialized structures that degrade the extracellular matrix (ECM) and promote cell invasion and metastasis. Understanding the forms and functions of invadopodia should facilitate the proper identification of novel targets for antiinvasive therapy. Methods To understand the role of insulin‐like growth factor‐II mRNA‐binding protein‐3 (IMP‐3) in invadopodia formation and cancer invasion, we performed IMP‐3 gene silencing, invadopodia formation, ECM degradation assay, zymography, western blot, and mouse xenograft. Results We demonstrate that invadopodia evidenced ECM degradation activity in oral squamous cell carcinoma (OSCC). Downregulation of IMP‐3 inhibited invadopodia formation, ECM degradation, and tumor growth and invasiveness. Epidermal growth factor receptor (EGFR) signaling may perform a critical function in invadopodia formation, ECM degradation, IMP‐3, and cortactin expression. Conclusion IMP‐3 may be intimately correlated with cancer invasion through invadopodia in oral cancer. The overexpression of IMP‐3 in oral cancer was predictive of a high correlation with cancer growth and invasion. © 2011 Wiley Periodicals, Inc. Head Neck, 2012