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Tumor microenvironmental genomic alterations in juvenile nasopharyngeal angiofibroma
Author(s) -
Silveira Sara Martoreli,
Domingues Maria Aparecida Custódio,
Butugan Ossamu,
Brentani Maria Mitzi,
Rogatto Silvia Regina
Publication year - 2012
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21767
Subject(s) - juvenile nasopharyngeal angiofibroma , biology , stromal cell , comparative genomic hybridization , gene , cancer research , microbiology and biotechnology , genetics , chromosome , medicine , radiology
Background To better characterize the pathophysiology of juvenile nasopharyngeal angiofibroma (JNA), endothelial and stromal cells were evaluated by genomic imbalances in association with transcript expression levels of genes mapped on these altered regions. Methods High‐resolution comparative genomic hybridization (HR‐CGH) was used in laser‐captured endothelial and stromal cells from 9 JNAs. Ten genes were evaluated by quantitative real‐timereverse transcription polymerase chain reaction (qRT‐PCR) in 15 cases. Results Although gains were more frequently detected in endothelial cells, 57% of chromosomal alterations were common by both components. Gene expression analyses revealed a positive correlation between endothelial and stromal components for ASPM , CDH1 , CTNNB1 , FGF18 , and SUPT16H . A significant difference was found for FGF18 and AURKB overexpression in stromal cells and AR down‐expression in endothelial cells. Conclusions A similar pattern of gene expression and chromosomal imbalances in both exponents would suggest a common mechanism of functional regulation. AURKB , FGF18 , and SUPT16H were identified as potential molecular markers in JNA. © 2011 Wiley Periodicals, Inc. Head Neck, 2012