Premium
Association between areca‐stimulated vimentin expression and the progression of head and neck cancers
Author(s) -
Tseng YuHsin,
Chang KuoWei,
Yang ChengChieh,
Liu ChungJi,
Kao ShouYen,
Liu TsungYun,
Lin ShuChun
Publication year - 2012
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21726
Subject(s) - areca , vimentin , medicine , immunohistochemistry , carcinoma , head and neck squamous cell carcinoma , cancer research , downregulation and upregulation , pathology , head and neck cancer , oncology , cancer , biology , nut , gene , biochemistry , structural engineering , engineering
Background Areca nut chewing is a common oral habit of Asians that is closely associated with the high incidence of head and neck carcinoma. The purpose of this study was to investigate the impacts of areca nut chewing on neoplastic process of head and neck carcinoma. Methods Head and neck carcinoma cells were treated with areca nut extract to perceive the phenotypic impacts. Tumor tissues were analyzed with immunohistochemistry (IHC) to understand the association between areca‐associated molecular changes and clinical variables. Results Upon treatment with areca nut extract, carcinoma cells showed the increase of vimentin. The activation of extracellular signal‐regulated kinase (ERK)/cyclooxygenase (COX)‐2/prostaglandin (PGE)‐2 cascade underlay the upregulation. These cells also exhibited the enhancement of migration and invasion. By knocking down COX‐2 and vimentin expression, the increase of cell mobility was reversed. Tumor exhibiting extensive vimentin and/or COX‐2 expression displayed a significantly worse disease‐associated survival than contrast groups. Conclusion Areca‐modulated vimentin expression enhanced the progression of head and neck carcinoma. © 2011 Wiley Periodicals, Inc. Head Neck , 2012