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Therapeutic effects of a fusogenic newcastle disease virus in treating head and neck cancer
Author(s) -
Li Pingdong,
Chen ChunHao,
Li Sen,
Givi Babak,
Yu Zhenkun,
Zamarin Dmitriy,
Palese Peter,
Fong Yuman,
Wong Richard J.
Publication year - 2011
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21609
Subject(s) - oncolytic virus , newcastle disease , multiplicity of infection , virus , virology , head and neck squamous cell carcinoma , biology , head and neck cancer , cell culture , green fluorescent protein , cancer research , cancer , gene , biochemistry , genetics
Background Newcastle disease virus (NDV) is a paramyxovirus that is pathogenic in birds but causes only mild flulike symptoms in human beings. NDV(F3aa)‐GFP is a genetically modified, fusogenic NDV. We assessed the utility of NDV(F3aa)‐GFP in treating head and neck squamous cell carcinoma. Methods and Results At a multiplicity of infection (MOI) of 1, NDV(F3aa)‐GFP infection of 3 cell lines supported strong GFP expression by 36 hours. Four cell lines were highly sensitivite to viral cytotoxicity, with >75% of cells lysed by day 6 at MOI 0.1, and 2 other cell lines were partially susceptible. Murine SCC25 flank tumors exhibited robust GFP expression after a single intratumoral viral injection and showed near‐complete tumor regression over 34 days. There were no adverse effects attributable to therapy. Conclusions We demonstrate that a fusogenic NDV exerts potent oncolytic effects against human head and neck cancer and support its continued investigation for clinical application. © 2010 Wiley Periodicals, Inc. Head Neck, 2010