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Increased prevalence of interleukin‐17–producing CD4 + tumor infiltrating lymphocytes in human oral squamous cell carcinoma
Author(s) -
Lee JangJaer,
Chang YenLiang,
Lai WanLing,
Ko JenqYuh,
Kuo Mark YenPing,
Chiang ChunPin,
Azuma Miyuki,
Chen ChingWen,
Chia JeanSan
Publication year - 2011
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21607
Subject(s) - basal cell , interleukin 2 , cancer research , medicine , tumor infiltrating lymphocytes , pathology , immunology , cytokine , cancer , immunotherapy
Background. T helper 17 (Th17) and regulatory T cells share plasticity in the expression of interleukin (IL)‐17 and forkhead box P3 (FOXP3), but their mutual presence in human diseases is unclear. Methods. IL‐17 and FOXP3 were analyzed by immunohistostaining and flow cytometry. The cytokine milieu was analyzed by quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR). Results. Oral squamous cell carcinoma expresses high levels of IL‐1β, IL‐6, and transforming growth factor (TGF)‐β. A unique subset of FOXP3 + IL‐17‐producing CD4 + T cells was consistently identified in tumor‐infiltrating lymphocytes from advanced stages of cancer, but not in the circulation, at a frequency of 0.5% to 5.5 % of total CD4 + T and positively correlated with the frequency of IL‐17 + FOXP3 − T cells. The IL‐17 + FOXP3 + T cells express CCR6 and suppress the proliferation of autologous CD4 + CD25 − responder T‐cells in vitro. Conclusions. The prevalence of IL‐17‐producing FOXP3 + CD4 + tumor infiltrating lymphocytes is increased in oral squamous cell carcinoma. © 2010 Wiley Periodicals, Inc. Head Neck, 2010