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Increased frequencies of CD4 + CD25 + FOXP3 + regulatory T cells in human nasal inverted papilloma
Author(s) -
Gu Yili,
Wang Chengshuo,
Han Demin,
Zhang Luo
Publication year - 2011
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21581
Subject(s) - foxp3 , il 2 receptor , nip , flow cytometry , microbiology and biotechnology , cytokine , medicine , chemistry , immunology , biology , cancer research , t cell , immune system , materials science , composite material
Background The purpose of this study was to investigate the presence of CD4 + CD25 + FOXP3 + regulatory T (Treg) cells both in peripheral blood and local tumors in patients with nasal inverted papilloma (NIP). Methods By using flow cytometry, the frequencies of CD4 + CD25 + FOXP3 + Treg cells in both peripheral blood and tissues from 18 patients with NIP and 8 control subjects were determined. CCL22 and CCL17 proteins in NIP tumors were analyzed by using enzyme‐linked immunosorbent assay. The suppressive capacity of Treg cells was estimated by WST‐8 and enzyme‐linked immunosorbent assay (ELISA; interferon‐gamma [IFN‐γ] and interleukin‐4 [IL‐4]) analysis. Results Patients with NIP showed increased CD4 + CD25 + FOXP3 + Treg cell frequencies in tumor tissues and CD4 + T cell fraction rather than in peripheral blood. CCL22 increased in NIP tumors. Phytohemagglutinin (PHA)‐induced proliferation and cytokine production of CD4 + CD25 ‐ T cells were suppressed equally well by CD4 + CD25 high cells from both patients with NIP and controls. Conclusion Our study demonstrated the increased frequencies of CD4 + CD25 + FOXP3 + Treg cells in NIP tumors, which might be influenced by CCL22. © 2010 Wiley Periodicals, Inc. Head Neck, 2011