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Evaluation of drug transporters' significance for multidrug resistance in head and neck squamous cell carcinoma
Author(s) -
Theile Dirk,
KetabiKiyanvash Nahal,
HeroldMende Christel,
Dyckhoff Gerhardt,
Efferth Thomas,
Bertholet Vincent,
Haefeli Walter Emil,
Weiss Johanna
Publication year - 2011
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21559
Subject(s) - paclitaxel , head and neck squamous cell carcinoma , multiple drug resistance , cisplatin , drug resistance , transporter , biology , cancer research , atp binding cassette transporter , reverse transcriptase , abcg2 , pharmacology , chemotherapy , cancer , head and neck cancer , polymerase chain reaction , gene , genetics
Background. Multidrug resistance (MDR) hampers chemotherapy in head and neck squamous cell carcinoma (HNSCC). There is little information about MDR mediating drug transporters in HNSCC. Methods. Nine HNSCC cell lines were characterized concerning drug transporter expression and susceptibility to cisplatin, paclitaxel, and 5‐fluorouracil (5‐FU) using a DNA microarray and proliferation assays. Three cells lines were precisely investigated for transporter expression using reverse transcriptase‐polymerase chain reaction (RT‐PCR) and drug resistance before and after simulated chemotherapies. Results. The cell lines differed in drug resistance and transporter expression. Cisplatin and paclitaxel resistances were inversely associated. Drugs rendered HNSCC cells resistant, cross‐resistant, or cross‐sensitive. Sensitivity changes were accompanied by transporter mRNA expression changes. Conclusion. Paclitaxel was identified as a potent inducer of numerous drug transporters and phenotypic MDR in HNSCC. Thus, cytostatic susceptibility of HNSCC cannot exclusively be deduced from the expression of single transporters but more likely of the entire transportome and non‐transporter modulators. © 2010 Wiley Periodicals, Inc. Head Neck, 2011