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Upregulation of interleukin‐1 by Epstein–Barr virus latent membrane protein 1 and its possible role in nasopharyngeal carcinoma cell growth
Author(s) -
Huang YuTzu,
Liu MeiYing,
Tsai ChingHwa,
Yeh TeHuei
Publication year - 2010
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21270
Subject(s) - nasopharyngeal carcinoma , transfection , biology , epstein–barr virus , tumor necrosis factor alpha , microbiology and biotechnology , virus , interleukin , downregulation and upregulation , messenger rna , cell culture , cancer research , virology , cytokine , immunology , gene , medicine , biochemistry , radiation therapy , genetics
Background Nasopharyngeal carcinoma (NPC) is associated with Epstein–Barr virus (EBV) infection. We previously found that interleukin (IL)‐1α and IL‐1β significantly increased in NPC tissues. This study investigated what EBV‐encoded proteins were involved in such IL‐1 production. Methods and Results IL‐1α and IL‐1β messenger ribonucleic acids (mRNAs) were detected in the EBV latent membrane protein 1 (LMP1) transfectant (LMP135) only by reverse transcriptase–polymerase chain reaction (RT‐PCR). LMP1‐mediated IL‐1α and IL‐1β production could be enhanced by tumor necrosis factor alpha (TNF‐α), determined by enzyme‐linked immunosorbent assay (ELISA). Moreover, IL‐1α and IL‐1β mRNAs and proteins were increased in a dose‐dependent manner in epithelial cells transiently transfected by an LMP1 plasmid. Besides, immortalized human epidermal keratinocyte (RHEK‐1) epithelial cells could be enhanced to proliferate by IL‐1α and IL‐1β determined by water‐soluble tetrazolium salt (WST‐1) assay. Conclusions EBV LMP1 is capable of upregulating IL‐1α and IL‐1β secretions from epithelial cells and positively modulated by TNF‐α. This may consequently contribute to tumor growth in patients with NPC. © 2009 Wiley Periodicals, Inc. Head Neck, 2009