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Dual priming oligonucleotide–based multiplex PCR analysis for detection of BRAF V600E mutation in FNAB samples of thyroid nodules in BRAF V600E mutation–prevalent area
Author(s) -
Kwak Jin Young,
Kim EunKyung,
Kim JongKee,
Han JeongHyun,
Hong Soon Won,
Park Tae Sung,
Choi Jong Rak
Publication year - 2010
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21210
Subject(s) - thyroid nodules , multiplex , medicine , multiplex polymerase chain reaction , v600e , thyroid cancer , thyroid carcinoma , thyroid , polymerase chain reaction , microbiology and biotechnology , pathology , mutation , biology , gene , genetics
Background. To evaluate the diagnostic value of dual priming oligonucleotide (DPO)–based multiplex polymerase chain reaction (PCR) for the detection of BRAF V600E mutations in ultrasound‐guided fine‐needle aspiration biopsy (US‐FNAB) of thyroid nodules. Methods. Our institutional review board approved this retrospective study, and informed consent was not required from patients. The 130 patients underwent US‐FNAB to evaluate BRAF status in thyroid nodules. In FNAB washouts, DPO‐based multiplex PCR, direct DNA sequencing, and PCR–restriction fragment length polymorphism (RFLP) were used to detect BRAF V600E . The diagnostic performance of these methods was calculated. We compared cytologic results by BRAF status. Results. Diagnostic accuracy and sensitivity were highest when screening with DPO‐based multiplex PCR. BRAF V600E positivity was a useful marker at thyroid nodules with “suspicious for papillary thyroid carcinoma” or “inadequate” cytological result. Conclusions. DPO‐based multiplex PCR may be an alternative to direct DNA sequencing because of its high sensitivity, high accuracy, and simplicity. BRAF V600E may be a useful additional diagnostic marker in BRAF V600E ‐prevalent areas. © 2009 Wiley Periodicals, Inc. Head Neck, 2010