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Inhibition of cell proliferation and glucose uptake in human laryngeal carcinoma cells by antisense oligonucleotides against glucose transporter‐1
Author(s) -
Zhou ShuiHong,
Fan Jun,
Chen XiaoMing,
Cheng KeJia,
Wang ShenQing
Publication year - 2009
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.21137
Subject(s) - glucose transporter , transfection , cell growth , microbiology and biotechnology , messenger rna , glucose uptake , in vitro , cell , in vivo , biology , gene expression , chemistry , antisense rna , gene , endocrinology , biochemistry , insulin
Background. Malignant cells show increased glucose uptake in vitro and in vivo, which is thought to be mediated by glucose transporters. In this study, we investigated the effect of plasmid‐derived antisense RNA against the Glut‐l gene on proliferation and glucose uptake in laryngeal carcinoma Hep‐2 cells. Methods. The expression plasmids pcDNA3.1(+)‐Glut‐1 and pcDNA3.1(+)‐anti Glut‐1 were constructed. The MTT method was used to assess cell growth inhibition. The expression of Glut‐1 mRNA and protein was detected by reverse transcriptase‐polymerase chain reaction and Western blotting, respectively. Results. After transfection, Glut‐1 AS clearly inhibited glucose uptake and cell growth in Hep‐2 cells, and we observed a decrease in the expression of Glut‐1 mRNA and protein in Hep‐2 cells. Conclusions. Glut‐1 AS decreases glucose uptake and inhibits the proliferation of Hep‐2 cells. © 2009 Wiley Periodicals, Inc. Head Neck, 2009