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In vivo investigation of CD133 as a putative marker of cancer stem cells in Hep‐2 cell line
Author(s) -
Wei Xu Dong,
Zhou Liang,
Cheng Lei,
Tian Jie,
Jiang Jack J.,
MacCallum Julia
Publication year - 2009
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.20935
Subject(s) - cancer stem cell , cell culture , cancer research , population , flow cytometry , biology , in vivo , stem cell , stem cell marker , cell , cell sorting , microbiology and biotechnology , medicine , genetics , environmental health
Background Mounting evidence suggests that most tumors consist of a heterogeneous population of cells with a subset population that has the exclusive tumorigenic ability. They are called cancer stem cells (CSCs). CSCs can self‐renew to generate additional CSCs and also differentiate to generate phenotypically diverse cancer cells with limited proliferative potential. They have been identified in a variety of tumors. In this study, we identify the marker of CSCs in the established human laryngeal tumor Hep‐2 cell line in vivo. Our in vitro experiment shown as CD133, a 5‐transmembrane glycoprotein expressed in Hep‐2 cell line. CD133 was supposed as a candidate of CSC in laryngeal carcinoma. In this study, the expression of CD133 was detected in a Hep‐2 cell line. Applying the magnetic cell sorting (MACS) technology, we reported the results of purifying CD133 positive cells from a Hep‐2 cell line. Three‐type cells' tumor‐forming ability was examined in vivo to identify the marker of CSCs in Hep‐2 cell line. Methods CD133 was selected as a putative marker of CSC in laryngeal carcinoma, Hep‐2 cell lines. Flow cytometry was used to detect the expression of CD133 in the Hep‐2 cell line. Immunomagnetic beads were applied to purify CD133‐positive cells. CD133(+), CD133(−) tumor cells, and unsorted Hep‐2 cells were injected into severe combined immune deficiency (SCID) mice individually to observe tumor‐forming ability. Results Only a small proportion (3.15% ± 0.83%) of cells in the Hep‐2 cell line express the CD133 marker. In comparison with CD133(−) tumor cells and unsorted cells, CD133(+) cells possess a marked capacity for tumor formation in vivo ( p <.05). Conclusion CD133 is 1 of the markers for CSCs in human laryngeal tumors of the Hep‐2 cell line. Work on the characterization of these cells provides a powerful tool to investigate the tumorigenic process in the larynx and to develop therapies targeting the CSC. © 2008 Wiley Periodicals, Inc. Head Neck, 2009