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Upregulation of discoidin domain receptor 2 in nasopharyngeal carcinoma
Author(s) -
Chua HueyHuey,
Yeh TeHuei,
Wang YingPiao,
Huang YuTzu,
Sheen TzungShiahn,
Lo YouChang,
Chou YaChing,
Tsai ChingHwa
Publication year - 2008
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.20724
Subject(s) - ddr1 , discoidin domain , nasopharyngeal carcinoma , downregulation and upregulation , transactivation , cancer research , transfection , receptor , chemistry , biology , receptor tyrosine kinase , medicine , gene expression , cell culture , gene , radiation therapy , biochemistry , genetics
Background. Nasopharyngeal carcinoma (NPC) is associated with Epstein‐Barr virus (EBV) and has high metastatic potential. Discoidin domain receptors (DDR1, DDR2) are receptor‐type tyrosine kinases activated by collagen. Their ability to induce expression of matrix metalloproteinase is related with tumor invasion. Therefore, we aim to investigate DDRs gene expression and its regulation in NPC. Methods and Results. By use of real‐time quantitative polymerase chain reaction (Q‐PCR), DDR2 gene expression but not DDR1 was significantly higher in primary and metastatic NPC. DDR2 was predominantly distributed in NPC tumor cells rather than in infiltrating lymphocytes. EBV Z‐transactivator (Zta) transfection may distinctly elevate DDR2 level. Furthermore, data from reporter assay indicate that Zta could transactivate DDR2 promoter activity, suggesting the possible upregulation mechanism. Conclusion. DDR2 was differentially upregulated in NPC and modulated by EBV Zta protein. DDR2 may play a role in NPC invasion and serve as a diagnostic and therapeutic target. © 2007 Wiley Periodicals, Inc. Head Neck, 2008