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Comprehensive genomic analysis identifies MDM2 and AURKA as novel amplified genes in juvenile angiofibromas
Author(s) -
Schick Bernhard,
Wemmert Silke,
Bechtel Ulrike,
Nicolai Piero,
Hofmann Thiemo,
Golabek Wieslaw,
Urbschat Steffi
Publication year - 2007
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.20535
Subject(s) - comparative genomic hybridization , metaphase , biology , genetics , gene , microarray , genome , microbiology and biotechnology , chromosome , gene expression
Background. Frequent β‐catenin mutations have been detected in juvenile angiofibromas, but the tumor pathogenesis remains unknown. Methods. Metaphase‐comparative genomic hybridization (CGH) was used to identify chromosomal aberrations in 29 tumor specimens. Two tumors were investigated using genome DNA microarrays. Results. Three hundred eleven chromosomal gains and losses were detected by metaphase‐CGH. Frequent chromosomal gains were detected at 4q, 6, 12, and X, while frequent chromosomal losses affected regions of chromosomes 8, 16, 17, 22, and Y. Genome DNA microarray analysis in 2 tumors of the series confirmed chromosomal aberrations, detected by metaphase‐CGH, and indicated genes such as AURKA (20q13.2) not being recognized by metaphase‐CGH. Conclusion. Metaphase‐CGH results confirmed numerous chromosomal aberrations in juvenile angiofibromas. The most frequent aberrations affected sex chromosomes. Further consensus regions of chromosomal aberrations were detected at 4q, 6, 8, 12, 16, 17, and 22. AURKA and MDM2 were identified as interesting novel amplified genes in juvenile angiofibromas. © 2006 Wiley Periodicals, Inc. Head Neck, 2007