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The effect of nimesulide, a selective cyclooxygenase‐2 inhibitor, on Ets‐1 and Ets‐2 expression in head and neck cancer cell lines
Author(s) -
Lamm Wolfgang,
Vormittag Laurenz,
Turhani Dritan,
Erovic Boban M.,
Czembirek Cornelia,
EderCzembirek Christina,
Thurnher Dietmar
Publication year - 2005
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.20285
Subject(s) - nimesulide , immunohistochemistry , carcinogenesis , cancer , head and neck cancer , cancer research , cell culture , cell , head and neck squamous cell carcinoma , medicine , pathology , chemistry , biology , biochemistry , genetics
Background. The protooncogenes Ets‐1 and Ets‐2 are involved in carcinogenesis of different tumors. Nimesulide, a selective cyclooxygenase‐2 (COX‐2) inhibitor, has antiproliferative effects on tumor cells. The question arises whether nimesulide influences Ets‐1 and Ets‐2 synthesis in head and neck tumors. Methods. Expression of Ets‐1 and Ets‐2 was analyzed in tumor tissues by immunohistochemistry. The influence of nimesulide and an extracellular signal‐regulated kinase (ERK) inhibitor on cell proliferation of two head and neck cancer cell lines and Ets‐1 and Ets‐2 expression was determined by automated cell counting and Western blotting, respectively. Results. Immunohistochemistry showed a high expression of Ets‐1 and Ets‐2 in tumor tissues. In both cell lines, Ets‐1 and Ets‐2 expression were reduced after 24 and 48 hours by nimesulide. Conclusion. Both Ets‐1 and Ets‐2 are overexpressed in head and neck cancer specimens. Inhibition of Ets‐1 and Ets‐2 expression in head and neck cancer cell lines by nimesulide might explain the proapoptotic property of this COX‐2 inhibitor. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005