Premium
Characterization of a spontaneously arising murine squamous cell carcinoma (SCC VII) as a prerequisite for head and neck cancer immunotherapy
Author(s) -
Khurana Dianne,
Martin Elizabeth A.,
Kasperbauer Jan L.,
O'Malley Bert W.,
Salomao Diva R.,
Chen Lieping,
Strome Scott E.
Publication year - 2001
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.1130
Subject(s) - immunotherapy , medicine , head and neck squamous cell carcinoma , cancer , cancer research , immunology , cancer immunotherapy , head and neck cancer , major histocompatibility complex , lung cancer , lewis lung carcinoma , pathology , antigen , immune system , metastasis
Background To develop novel therapeutic approaches for patients with head and neck malignancies, poorly immunogenic murine models of squamous cell carcinoma (SCC) need to be defined. Methods The phenotype, growth characteristics, and responsiveness to tumor‐specific T‐cell transfer of a spontaneously arising murine SCC (SCC VII) were characterized. Results SCC VII expresses major histocompatibility complex (MHC) class I molecules yet is resistant to tumor‐specific T‐cell killing and relatively insensitive to killing mediated by lymphokine‐activated killer (LAK) cells. Intradermal tumors are reproducibly established after vaccination of 5 × 10 4 cells, and systemic micrometastases are apparent after intravenous administration of 2.5 × 10 4 cells. Immunotherapy of 3‐day lung metastases using tumor‐specific T cells and systemic interleukin‐2 (IL‐2) was ineffective in reducing the number of metastases in vivo. Conclusions SCC VII is a poorly immunogenic murine squamous cell cancer, which represents an ideal model for preclinical testing of immunotherapeutic approaches for patients with SCC of the upper aerodigestive tract. © 2001 John Wiley & Sons, Inc. Head Neck 23: 899–906, 2001.