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Platelet a disintegrin and metallopeptidase 10 expression correlates with clock drawing test scores in Alzheimer's disease
Author(s) -
Manzine Patrícia Regina,
Barham Elizabeth Joan,
Vale Francisco Assis Carvalho,
SelistredeAraújo Heloisa Sobreiro,
Pavarini Sofia Cristina Iost,
Cominetti Márcia Regina
Publication year - 2014
Publication title -
international journal of geriatric psychiatry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 129
eISSN - 1099-1166
pISSN - 0885-6230
DOI - 10.1002/gps.4020
Subject(s) - adam10 , platelet , medicine , disintegrin , alzheimer's disease , analysis of variance , disease , psychology , oncology , gastroenterology , metalloproteinase , matrix metalloproteinase
Objective Earlier studies have demonstrated that a disintegrin and metallopeptidase 10 (ADAM10) levels are reduced in Alzheimer's disease (AD) patients compared with healthy subjects. The objective of this study was to evaluate whether platelet ADAM10 levels correlates with the clock drawing test (CDT) scores, which is a simple and a reliable measure of visuospatial ability and executive function in AD patients. Methods Thirty elderly patients with probable AD and 25 healthy patients forming the control group, matched by age, gender, and educational level, were evaluated. Platelet proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and ADAM10 was identified by western blotting. The Spearman's correlation coefficient between ADAM10 and CDT was obtained for each group. The areas under the curves were used to compare the receiver operating characteristic curves. Results The CDT scores and platelet ADAM10 expression were significantly different between patients with AD and controls and also along the disease's progression. In AD patients, there was a positive correlation between ADAM10 expression and CDT scores. Among non‐AD subjects, no correlation was found. The combination of ADAM10 and CDT was significantly better to confirm the AD diagnosis than the AUCs of ADAM10 and CDT separately. Conclusions The association of blood‐based biomarkers, such as ADAM10, and cognitive tests may be helpful for a more reliable AD diagnosis. Copyright © 2013 John Wiley & Sons, Ltd.