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Calcium signaling in cultured rat oligodendrocytes
Author(s) -
Takeda Margaret,
Nelson Deborah J.,
Soliven Betty
Publication year - 1995
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440140308
Subject(s) - biology , oligodendrocyte , thapsigargin , calcium in biology , microbiology and biotechnology , carbachol , calcium signaling , intracellular , glutamate receptor , neuroglia , calcium , neuroscience , receptor , central nervous system , medicine , biochemistry , myelin , stimulation
The syntax of neuronal‐glial or axonal‐glial interaction is frequently communicated through transient changes in internal calcium (Ca i ). We examined mechanisms for Ca i signaling and intercellular propagation of Ca i responses in cultured oligodendrocytes (OLGs) derived form adult spinal cord (SC), postnatal day 21 (P21) SC, and P21 brain. We found that (1) cultured OLGs exhibited a heterogeneous responese to norepinephrine, carbachol, ATP, histamine, and glutamate; (2) receptor‐mediated Ca i increases were derived from both Ca 2+ influx and intracellular Ca 2+ release; (3) the percentage of responders to neuroligands varied as a function of cell origin; (4) cultured OLGs exhibited a thapsigargin‐sensitive, but not a caffeine‐sensitive, intracellular Ca 2+ pool; and (5) gap junctional contacts between OLGs permitted limited intercellular propagation of mechanically stimulated Ca i responses. Receptor‐mediated Ca i signaling appears to occur not only in cultured OLGs but also in acutely dissociated OLGs. The heterogeneity in Ca i responses as a function of cell origin may reflect the existence of OLG subsets of differences in the maturation stage of OLGs. © 1995 Wiley‐Liss, Inc.