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Excitatory amino acid receptors in glial progenitor cells: Molecular and functional properties
Author(s) -
Gallo Vittorio,
Patneau Doris K.,
Mayer Mark L.,
Vaccarino Flora M.
Publication year - 1994
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440110204
Subject(s) - kainate receptor , ampa receptor , cnqx , biology , glutamate receptor , microbiology and biotechnology , nmda receptor , oligodendrocyte , neuroscience , excitatory postsynaptic potential , neuroglia , kainic acid , receptor , biochemistry , inhibitory postsynaptic potential , central nervous system , myelin
We have analyzed the molecular and biophysical properties of glutamate‐gated channels in cells of the oligodendrocyte lineage, using both the CG‐4 primary cell line (Louis et al: J. Neurosci. Res. 31:193‐204, 1992a) and oligodendrocyte progenitors purified from the rat cerebral cortex. CG‐4 progenitor cells, as well as primary progenitors, were stained with a specific anti‐GABA antibody. In whole‐cell patch‐clamp recordings, rapid perfusion of the agonists L‐glutamate, kainate, and AMPA produced rapidly desensitizing currents in CG‐4 cells. NMDA was ineffective. Both rapidly desensitizing and steady‐state components of responses to kainate were inhibited by the kainate/AMPA receptor antagonist CNQX. Northern blot analysis of total mRNA isolated from CG‐4 cells revealed co‐expression of both AMPA‐ and kainate‐preferring glutamate receptor subunits. The activation of glutamate receptors in CG‐4 cells caused a rapid and transient elevation of mRNAs for the immediate early gene NGFI‐A. © 1994 Wiley‐Liss, Inc.