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Cloned microglial cells but not macrophages synthesize β‐endorphin in response to CRH activation
Author(s) -
Sacerdote Paola,
DenisDonini Suzanne,
Paglia Paola,
Granucci Francesca,
Panerai Alberto E.,
RicciardiCastagnoli Paola
Publication year - 1993
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440090408
Subject(s) - microglia , biology , lipopolysaccharide , macrophage , microbiology and biotechnology , cell culture , neuroglia , neurotransmitter , cell type , cell , immunology , neuroscience , central nervous system , inflammation , in vitro , biochemistry , genetics
The properties of microglial cell clones, obtained from embryonic mouse brain primary cultures immortalized with recombinant retroviruses, have been investigated and compared with the properties of macrophage clones similarly obtained. Macrophage clones differed from microglial clones in some functions but shared most of the immunological properties. Interestingly, microglial cells were able to produce β‐endo‐rphin, and this production was regulated differently in microglial cell clones when compared with macrophage clones. Although lipopolysaccharide (LPS) treatment induces an increase in β‐endorphin concentration in both cell types, only microglial clones and primary microglial cell cultures respond to the neuroendocrine stimulus corticotropin releasing hormone (CRH). In addition, in these cells, β‐endorphin release is regulated by a classical neurotransmitter, such as noradrenaline, adding some evidence of communication between neurons and microglial cells. © 1993 Wiley‐Liss, Inc.