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Thyroid hormone up‐regulates thyroid hormone receptor β gene expression in rat cerebral hemisphere astrocyte cultures
Author(s) -
Lebel JeanMarc,
L'Hérault Sylvie,
Dussault Jean H.,
Puymirat Jack
Publication year - 1993
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440090203
Subject(s) - astrocyte , thyroid hormone receptor , biology , receptor , thyroid , endocrinology , medicine , triiodothyronine , hormone , gene expression , thyroid hormone receptor beta , thyroid hormone receptor alpha , microbiology and biotechnology , hormone receptor , nuclear receptor , gene , central nervous system , biochemistry , transcription factor , genetics , cancer , breast cancer
Oligonucleotide probes complementary to specific regions of three thyroid receptor cDNAs were used to study the effects of thyroid hormone on the expression of the mRNAs encoding two α (α1 and α2) and one β‐thyroid (β1) receptors isoforms in rat cerebral hemisphere astrocyte cultures. Both genes are expressed by type 1 astrocytes. The levels of the α1‐, α2‐, and β1‐mRNAs did not significantly change between day 8 and day 22, in cultures grown in the absence of thyroid hormone. L‐triiodothyronine (L‐T3) treatment of the cultures increased the levels of β1‐mRNAs by fivefold without changing either the levels of the α1‐ and α2‐mRNAs or L‐T3 binding capacity. The effect of L‐T3 on β1‐mRNAs was observed after 4 h of treatment and was independent of protein synthesis, suggesting that this effect is likely to be a direct one. Treatment of the cultures by cytosine arabinosine, a drug that kills dividing cells, specifically decreased level of the α1‐ and α2‐mRNAs by 60% and 38%, respectively. Finally, by immunocytochemistry, we showed that the β1 receptor‐immunoreactivity was either located in the perinuclear region and the cytoplasm or in the nuclei of astrocytes. Taken together with previous data obtained in neuronal cultures where no effect of L‐T3 was observed on the levels of the β1‐mRNAs, our findings indicate that the β1 gene is differentially regulated in neurons and astrocytes. Furthermore, our results are also in favor of specific functions for both types of thyroid receptors during astrocyte differentiation. © 1993 Wiley‐Liss, Inc.