Premium
Differential expression of carbonic anhydrase isozymes in microglial cell types
Author(s) -
NóGrádi Antal
Publication year - 1993
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440080209
Subject(s) - biology , carbonic anhydrase , colocalization , isozyme , central nervous system , griffonia simplicifolia , carbonic anhydrase i , spinal cord , microglia , neuroglia , immunohistochemistry , nervous system , microbiology and biotechnology , carbonic anhydrase ii , pathology , biochemistry , neuroscience , immunology , enzyme , inflammation , medicine
Abstract Microglial cells have been shown to express carbonic anhydrase. Using carbonic anhydrase histochemistry and immunohistochemistry, different types of cen‐tral nervous system microglial cells were detected, which expressed two main carbonic anhydrase (CA) isozymes during the early postnatal stage of development and after peripheral nerve injury in the spinal cord of adult rats. Amoeboid and reactive microglial cells were heavily immunostained for CA‐II and CA‐III and showed colocalization with complement receptor type 3 and Griffonia Simplicifolia B 4 isolectin. Resting microglial cells in the brain and spinal cord showed faint CA‐III staining and were negative for CA‐II. These results show that not only CA‐II, but also CA‐III isozyme is represented in the central nervous system and carbonic anhydrase activity may correlate with metabolic and immunological changes of microglial cells. These data also further strengthen the idea of the mesodermal origin of central nervous system macrophages. © 1993 Wiley‐Liss, Inc.