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Appearance of GFAP‐positive cells in adult human brain cultures spontaneously decelerated in growth
Author(s) -
Peržel'Ova Anna,
Mareš Vladislav
Publication year - 1993
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440070307
Subject(s) - vimentin , glial fibrillary acidic protein , biology , gfap stain , explant culture , cell culture , astrocyte , staining , pathology , microbiology and biotechnology , neuroglia , cell growth , immunohistochemistry , in vitro , immunology , endocrinology , central nervous system , biochemistry , genetics , medicine
Cell cultures were derived from adult human brain biopsies [from cortical gray (cultures 9‐HB‐G and 33‐HB‐G) and white (culture 14‐HB‐W) and stroke‐injured white matter (culture 33‐HB‐IW)]. The morphology and growth rate of cultured cells were examined and correlated with the presence of vimentin and glial fibrillary acidic protein (GFAP). The cultures from various brain matters differed in cell morphology and rate of growth but not in GFAP and vimentin staining. Cells of primary and rapidly proliferating cultures were GFAP‐negative and vimentin‐positive. Spontaneous growth deceleration occurred in culture 14‐HB‐W within passages 5 to 10 and in cultures 9‐HB‐G, 33‐HB‐G, and 33‐HB‐W within passages 17 to 20. This deceleration, as well as the successive complete growth arrest, were accompanied by an appearance of GFAP‐positive cells and an elevated intensity for vimentin staining. We propose that GFAP‐positive astrocytes originate from glial precursor cells that migrate from the explants and differentiate under prolonged subcultivation.