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Effects of transforming growth factor‐β1 on the extracellular matrix and cytoskeleton of cultured astrocytes
Author(s) -
Baghdassarian Denise,
ToruDelbauffe Daniele,
Gavaret Jean Michel,
Pierre Michel
Publication year - 1993
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440070302
Subject(s) - extracellular matrix , fibronectin , cytoskeleton , laminin , microbiology and biotechnology , biology , glial fibrillary acidic protein , transforming growth factor , actin , transforming growth factor beta , immunocytochemistry , cell , immunology , biochemistry , endocrinology , immunohistochemistry
Abstract The present study was performed on primary cultures and subcultures of cerebellar astrocytes in order to investigate the effects of transforming growth factor‐β1 (TGFβ1) on proliferation, extracellular matrix (ECM) components, and cytoskeletal structures in relation to morphological changes. The expression and cellular distribution of the ECM components laminin and fibronectin and the cytoskeletal proteins glial fibrillary acidic protein (GFAP) and actin were investigated by immunoblotting, immunocytochemistry, and phalloidin staining. The proliferation of primary cultures was strongly inhibited by TGFβ1. Treated cells became enlarged and spread onto the substratum. TGFβ1 promoted the appearance of actin stress fibers and increased the cell actin content. It elicited a slight increase in GFAP expression and induced dispersion of thin filaments of GFAP. TGFβ1 also stimulated the production of laminin and fibronectin and their incorporation into the ECM of primary cultures grown in medium with or without serum Astrocytes grown in serum‐containing medium for 1 day after subculturing responded strongly to TGFβ1. Changes promoted by TGFβ1 in cell shape, cytoskeleton, and ECM production of cultured astrocytes may have relevance for understanding the mechanisms of action of TGFβ1 during brain development.