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Effect of insulin of glucose and glycogen metabolism and leucine incorporation into protein in cultured mouse astrocytes
Author(s) -
Kum W.,
Zhu S. Q.,
Ho S. K. S.,
Young J. D.,
Cockram C. S.
Publication year - 1992
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440060404
Subject(s) - insulin , glycogen , biology , medicine , endocrinology , stimulation , astrocyte , basal (medicine) , glycogen synthase , leucine , glucose uptake , metabolism , insulin receptor , carbohydrate metabolism , biochemistry , amino acid , insulin resistance , central nervous system
Insulin, following binding to its receptor, produces a dose‐ and time‐dependent stimulation of entry of 2‐deoxy‐D‐[U‐ 14 C] glucose and glycogen synthesis from D‐[U‐ 14 C] glucose in cultured mouse astrocytes following differentiation. Maximal stimulation of both glucose entry (217% above basal) and of glycogen synthesis (209% above basal) was observed at an insulin concentration of 1.7 × 10 −8 M. Insulin also stimulates the incorporation of leucine into astrocytic proteins with maximal stimulation (156% above basal) at an insulin concentration of 1.7 × 10 −7 M, but no effect on leucine uptake was observed at an insulin concentration of 1.7 × 10 −6 M. These results, together with a previous demonstration that insulin and certain insulin analogues stimulate pyrimidine nucleoside incorporation into nucleic acid, indicate that insulin has diverse actions on biomacromolecular metabolism in cultured mouse astrocytes. © 1992 Wiley‐Liss, Inc.