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Isolated growth cones stimulate proliferation of cultured Schwann cells
Author(s) -
Dent Erik W.,
Ida James A.,
Yoshino Jun E.
Publication year - 1992
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440050204
Subject(s) - axolemma , biology , growth cone , schwann cell , microbiology and biotechnology , cell growth , electron microscope , endocrinology , biochemistry , central nervous system , myelin , axon , physics , optics
A growth cone‐enriched fraction was prepared from 3–4 day rat cerebra. Examination of the growth cone fraction by electron microscopy revealed numerous structures circular in appearance that contain a number of features common to neuronal growth cones in vivo. The isolated growth cones stimulated a dose‐dependent incorporation of [ 3 H]‐thymidine into cultured Schwann cells in a manner similar to that observed with an axolemma‐enriched fraction prepared from adult rat brainstem. The mitogenic activities of both the growth cone fraction and axolemma‐enriched fraction were decreased 50% and 20%, respectively, by treatment with heparitinase I. The mitogen for Schwann cells present in the isolated growth cones appears to be similar to that found in axolemma‐enriched fractions prepared from adult rats.