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DBcAMP effect on the expression of GFAP and of its encoding mRNA in astroglial primary cultures
Author(s) -
Le Prince G.,
Fages C.,
Rolland B.,
Nunez J.,
Tardy M.
Publication year - 1991
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440040310
Subject(s) - glial fibrillary acidic protein , biology , messenger rna , astrocyte , gfap stain , microbiology and biotechnology , protein biosynthesis , cell culture , medicine , endocrinology , immunohistochemistry , immunology , gene , biochemistry , genetics , central nervous system
Short term and chronic dBcAMP effects on the expression of glial fibrillary acidic protein (GFAP) in astroglial primary cultures are investigated. Short (48 h) and long (more than 7 days) treatments with the cAMP derivative induce both cell shape changes and an increase in GFAP immunolabelling. Such effects are only associated with an increase in GFAP and in GFAP‐mRNA levels in the long term treatment. These results suggest that the short term effect of dBcAMP induces post‐translational modifications of the protein whereas the long term effect is associated with an increase in GFAP mRNA transcription and/or stability.