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Morphological changes of astroglia in the cerebellar cortex of developing mice after neonatal administration of cytosine arabinoside
Author(s) -
Ono Katsuhiko,
Mizukawa Kiminao,
Yanagihara Mamoru,
Tokunaga Akira
Publication year - 1990
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440030411
Subject(s) - cerebellum , biology , glial fibrillary acidic protein , pathology , cytosine , cerebellar cortex , immunohistochemistry , cortex (anatomy) , hypoplasia , granular layer , anatomy , neuroscience , immunology , biochemistry , medicine , dna
Neonatal administration of cytosine arabinoside (Ara‐C) induced marked hypoplasia of the mouse cerebellum. In 17–20‐day‐old mice with neonatal Ara‐C injections, the external granular layer was still preserved as an irregular cell laminae in the superficial part of the cerebellum, in contrast to its complete disappearance in normal animals at these stages. Immunohistochemical examination using anti‐glial fibrillary acidic protein and monoclonal antibody‐1D11 (Ono et al: Dev. Brain Res. , 50:154–159, 1989) demonstrated malformation of a palisade‐like arrangement of labeled glial fibers in the superficial part of the Ara‐C treated mouse cerebellum. Furthermore, the immunoreactive astroglia were observed just beneath the pial membrane and their processes were oriented to the deeper part of the molecular layer.