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Expression of glial fibrillary acidic protein (GFAP) in goldfish optic nerve following injury
Author(s) -
Stafford C. A.,
Shehab S. A. S.,
a S. N.,
CronlyDillon J. R.
Publication year - 1990
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440030106
Subject(s) - glial fibrillary acidic protein , optic nerve , biology , crush injury , regeneration (biology) , immunohistochemistry , neuroscience , anatomy , glial scar , microbiology and biotechnology , astrocyte , pathology , central nervous system , immunology , medicine , surgery
By using an antibody to goldfish glial fibrillary acidic protein (GFAP), the reaction of goldfish optic nerve to injury has been studied by immunoblotting and immunohistochemical methods. Goldfish optic nerve, which normally lacks GFAP immunoreactivity (Nona et al.: Glia , 2:189–200, 1989), expresses GFAP following injury. This immunoreactivity, which is observed as early as 10 days after crush and which is still evident at 30 days after crush, all but disappears by 150 days after crush. Since it is well established that functional restoration of synaptic connections and the recovery of vision takes place in goldfish following optic nerve injury, our results indicate that reactive astrocytes do not represent an impediment to regeneration in goldfish visual system.