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Glial fibrillary acidic protein (GFAP) from goldfish: Its localisation in visual pathway
Author(s) -
a S.N.,
Shehab S.A.S.,
Stafford C.A.,
CronlyDillon J.R.
Publication year - 1989
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440020308
Subject(s) - biology , glial fibrillary acidic protein , gfap stain , optic tract , polyclonal antibodies , retina , tectum , optic nerve , intermediate filament , vertebrate , microbiology and biotechnology , immunohistochemistry , anatomy , central nervous system , antibody , neuroscience , biochemistry , midbrain , cell , cytoskeleton , immunology , gene
An intermediate filament fraction, isolated from goldfish brain, contains a prominent protein having a molecular weight of 51 kDa. In normal goldfish visual pathway, this protein is present in tectum and tract, but not in optic nerve. A polyclonal antibody raised to this protein clearly labels ependymal glial profiles in tectum and parallel processes in the tract, whereas optic nerve is unlabelled; Müller fibres in the retina are also labelled. A similar, but less prominent, pattern of staining is observed with antibodies, raised elsewhere, against glial fibrillary acidic protein from human and porcine. These results suggest that the 51 kDa protein is a GFAP, demonstrate the heterogeneity of astrocytes in goldfish visual pathway, and are consistent with the idea that GFAP is well conserved in vertebrate phylogeny.
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