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Glutamate and kainate increase intracellular sodium activity in leech neuropile glial cells
Author(s) -
Ballanyi K.,
Dörner R.,
Schlue W.R.
Publication year - 1989
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440020106
Subject(s) - kainate receptor , hirudo medicinalis , leech , glutamate receptor , biology , membrane potential , nmda receptor , hyperpolarization (physics) , neuroscience , biophysics , intracellular , ampa receptor , biochemistry , chemistry , receptor , stereochemistry , world wide web , computer science , nuclear magnetic resonance spectroscopy
Na + ‐selective, double‐barrelled microelectrodes were used to measure intracellular Na + activity (a i Na ) and membrane potential (Em) in neuropile glial cells of isolated segmental ganglia in the leech Hirudo medicinalis . Bath application of glutamate (10 −3 M) resulted in membrane depolarizations of about 5 mV and a concomitant increase of a i Naby between 2 and 10 mM. Kainate (10 −4 M) elicited depolarizations of up to 40 mV amplitude followed by a prominent afterhyperpolarization. During kainate, a i Naincreased by 7 to 25 mM. In contrast to glutamate, an initial decrease of a i Nawas detected during the action of kainate. N‐methyl‐D‐aspartate (NMDA, 10 −5 –10 −3 M) had no effect of Em and a i Na . The results indicate that leech glial cells have a kainate‐preferring non‐NMDA glutamate receptor.