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Direct effects of carbachol on membrane potential and ion activities in leech glial cells
Author(s) -
Ballanyi K.,
Schlue W.R.
Publication year - 1988
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440010209
Subject(s) - leech , depolarization , carbachol , biology , hirudo medicinalis , biophysics , membrane potential , ion channel , neuroscience , extracellular , neuroglia , microbiology and biotechnology , biochemistry , receptor , central nervous system , stimulation , world wide web , computer science
Ion‐selective double‐barreled microelectrodes were used to measure the activities of intracellular K + and Na + (a i k , a i Na ) and the membrane potential (E m ) in neuropile glial cells as well as extracellular K + (a e K ) in the neuropile of segmental ganglia in the leech, Hirudo medicinalis . Bath‐application of carbachol resulted in a prominent membrane depolarization. This depolarization was accompanied by transient increases of a i Naand a e K , whereas a i Kdecreased. It is suggested that the carbachol‐induced depolarization and the underlying ion activity changes are due to activation of an acetylcholine receptor‐coupled cation channel in the membrane of the neuropile glial cells.