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Structural complexity of primary cultures of astrocytes as revealed by transverse sections
Author(s) -
Devon Richard M.,
Juurlink Bernhard H. J.
Publication year - 1988
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440010207
Subject(s) - astrocyte , biology , transverse plane , electron microscope , monolayer , biophysics , intracellular , microbiology and biotechnology , anatomy , optics , physics , neuroscience , biochemistry , central nervous system
The tissue architecture of low‐, medium‐, and high‐density primary mouse astroglial cultures was examined in horizontal and transverse planes using the electron microscope. It was found that the low‐density (colony) cultures consisted of a true monolayer, whereas the medium‐ and high‐density (confluent) cultures consisted of anywhere from two to seven overlapping sheets enclosing a substantial intercellular space. The presence of these multiple overlapping sheets in confluent astrocyte cultures should therefore be taken into consideration when interpreting data of cell‐membrane‐related phenomena such as ion fluxes.