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Growth factors for human glial cells in culture
Author(s) -
Yong Voon Wee,
Kim Seung U.,
Kim Myong W.,
Shin Doo H.
Publication year - 1988
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.440010203
Subject(s) - biology , neuroglia , platelet derived growth factor , growth factor , basic fibroblast growth factor , nerve growth factor , astrocyte , microbiology and biotechnology , fetal bovine serum , cell culture , fibroblast growth factor , immunology , medicine , endocrinology , platelet derived growth factor receptor , central nervous system , biochemistry , receptor , genetics
Using a new double‐labeling immunofluorescence technique, we assessed various growth factors on their ability to promote proliferation of cultured human glial cells. Cells studied were fetal astrocytes, fetal Schwann cells, adult astrocytes, and adult oligodendrocytes. Effective agents for fetal astrocytes were glial growth factor from the bovine pituitary, platelet‐derived growth factor, fibroblast growth factor, and 4β‐phorbol 12, 13‐dibutyrate. For fetal Schwann cells, mitogens were glial growth factor from the bovine pituitary, platelet‐derived growth factor, nerve growth factor, and 4β‐phorbol 12, 13‐dibutyrate. Adult astrocytes and oligodendrocytes did not normally divide in culture, and none of the agents tested were effective in inducing their proliferation. The report that interleukin‐2 was a mitogen for oligodendrocytes could not be replicated in the present study on any of the glial cell types.