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Neuronal lactate levels depend on glia‐derived lactate during high brain activity in Drosophila
Author(s) -
GonzálezGutiérrez Andrés,
Ibacache Andrés,
Esparza Andrés,
Barros Luis Felipe,
Sierralta Jimena
Publication year - 2020
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.23772
Subject(s) - biology , neuroglia , neuroscience , monocarboxylate transporter , premovement neuronal activity , microbiology and biotechnology , ependymal cell , neuron , in vivo , transporter , astrocyte , electrophysiology , central nervous system , biochemistry , gene
Lactate/pyruvate transport between glial cells and neurons is thought to play an important role in how brain cells sustain the high‐energy demand that neuronal activity requires. However, the in vivo mechanisms and characteristics that underlie the transport of monocarboxylates are poorly described. Here, we use Drosophila expressing genetically encoded FRET sensors to provide an ex vivo characterization of the transport of monocarboxylates in motor neurons and glial cells from the larval ventral nerve cord. We show that lactate/pyruvate transport in glial cells is coupled to protons and is more efficient than in neurons. Glial cells maintain higher levels of intracellular lactate generating a positive gradient toward neurons. Interestingly, during high neuronal activity, raised lactate in motor neurons is dependent on transfer from glial cells mediated in part by the previously described monocarboxylate transporter Chaski, providing support for in vivo glia‐to‐neuron lactate shuttling during neuronal activity.