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TGF‐β pro‐oligodendrogenic effects on adult SVZ progenitor cultures and its interaction with the Notch signaling pathway
Author(s) -
Gómez Pinto Laura I.,
Rodríguez Debora,
Adamo Ana M.,
Mathieu Patricia A.
Publication year - 2018
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.23253
Subject(s) - subventricular zone , notch signaling pathway , biology , hes1 , progenitor cell , nestin , microbiology and biotechnology , transforming growth factor , neural stem cell , olig2 , glial fibrillary acidic protein , neurogenesis , growth factor , signal transduction , stem cell , neuroscience , oligodendrocyte , receptor , immunology , central nervous system , immunohistochemistry , myelin , biochemistry
Adult neural progenitor cells (NPCs) are capable of differentiating into neurons, astrocytes, and oligodendrocytes throughout life. Notch and transforming growth factor β1 (TGF‐β) signaling pathways play critical roles in controlling these cell fate decisions. TGF‐β has been previously shown to exert pro‐neurogenic effects on hippocampal and subventricular zone (SVZ) NPCs in vitro and to interact with Notch in different cellular types. Therefore, the aim of our work was to study the effect of TGF‐β on adult rat brain SVZ NPC glial commitment and its interaction with Notch signaling. Initial cell characterization revealed a large proportion of Olig2+, Nestin+, and glial fibrillary acidic protein (GFAP+) cells, a low percentage of platelet‐derived growth factor receptor α (PDGFRα+) or NG2+ cells, and <1% Tuj1+ cells. Immunocytochemical analyses showed a significant increase in the percentage of PDGFRα+, NG2+, and GFAP+ cells upon four‐day TGF‐β treatment, which demonstrates the pro‐gliogenic effect of this growth factor on adult brain SVZ NPCs. Real‐time polymerase chain reaction analyses showed that TGF‐β induced the expression of Notch ligand Jagged1 and downstream gene Hes1. Notch signaling inhibition in cultures treated with TGF‐β produced a decrease in the proportion of PDGFRα+ cells, while TGF‐β receptor II (TβRII) inhibition also rendered a decrease in the proportion of PDGFRα+ cells, concomitantly with a decrease in Jagged1 levels. These findings demonstrate the participation of Notch signaling in TGF‐β effects and illustrate the impact of TGF‐β on glial cell fate decisions of adult brain SVZ NPCs, as well as on oligodendroglial progenitor cell proliferation and maturation.