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Polarized distribution of AMPA, but not GABA A , receptors in radial glia‐like cells of the adult dentate gyrus
Author(s) -
Renzel Roland,
Sadek AhmedRamadan,
Chang CinHe,
Gray William P.,
Seifert Gerald,
Steinhäuser Christian
Publication year - 2013
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.22505
Subject(s) - ampa receptor , glutamate receptor , ionotropic effect , biology , dentate gyrus , kainate receptor , neurogenesis , neuroscience , microbiology and biotechnology , neuroglia , receptor , long term depression , hippocampus , biochemistry , central nervous system
Glial fibrillary acidic protein (GFAP)‐positive astrocytes with radial processes [radial glia (RG)‐like cells] in the postnatal dentate gyrus share many of the characteristics of embryonic radial glia and appear to act as precursor cells for adult dentate neurogenesis, a process important for pattern separation and hippocampus‐dependent learning. Although much work has delineated the mechanisms underlying activity‐neurogenesis coupling via gamma‐amino butyric acid (GABA)ergic neurotransmission on GFAP‐negative transient‐amplifying cells and neuroblasts, little is known regarding the effects of neurotransmitters on RG‐like cells. Conflicting evidence exists for both GABA and glutamate receptors on these cells. Here, using GFAP reporter mice, we show that the somatic membrane of RG‐like cells carries GABA A receptors and glutamate transporters but not ionotropic glutamate receptors, whereas 2‐amino‐3‐(hydroxyl‐5‐methylisoxazole‐4‐yl) propionic acid (AMPA) and GABA A receptors are expressed on the processes of these cells. Almost all RG‐like cells expressed the GluA2 subunit, which restricts the Ca 2+ permeability of AMPA receptors. The glial GABA A receptors mainly comprised α2/α4, β1, and γ1/γ3. The selective presence of AMPA receptors on the radial processes may be important for sensing and responding to local activity‐driven glutamate release and supports the concept that RG‐like astrocytes are composed of functional and structural domains.