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A proteome map of axoglial specializations isolated and purified from human central nervous system
Author(s) -
Dhaunchak Ajit S.,
Huang Jeffrey K.,
De Faria Omar,
Roth Alejandro D.,
Pedraza Liliana,
Antel Jack P.,
BarOr Amit,
Colman David R.
Publication year - 2010
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.21064
Subject(s) - myelin , proteome , multiple sclerosis , biology , node of ranvier , proteomics , white matter , neuroscience , central nervous system , bioinformatics , genetics , immunology , gene , medicine , radiology , magnetic resonance imaging
Compact myelin, the paranode, and the juxtaparanode are discrete domains that are formed on myelinated axons. In humans, neurological disorders associated with loss of myelin, including Multiple Sclerosis, often also result in disassembly of the node of Ranvier. Despite the importance of these domains in the proper functioning of the CNS, their molecular composition and assembly mechanism remains largely unknown. We therefore performed a large‐scale proteomics MudPIT screen for the identification of proteins in human myelin and axogliasomal fractions. We identified over 1,000 proteins in these fractions. Since even minor perturbations in neuron‐glial interactions can uncouple the glial support of axons, the proteome map presented here can be used as a reference library for “myelin health” and disease states, including white matter disorders such as leukodystrophies and multiple sclerosis. © 2010 Wiley‐Liss, Inc.