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Different astroglia permissivity controls the migration of olfactory bulb interneuron precursors
Author(s) -
GarcíaMarqués Jorge,
De Carlos Juan A.,
Greer Charles A.,
LópezMascaraque Laura
Publication year - 2009
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20918
Subject(s) - olfactory bulb , rostral migratory stream , biology , neuroblast , interneuron , astrocyte , cell migration , neuroscience , extracellular matrix , microbiology and biotechnology , cell , subventricular zone , central nervous system , neural stem cell , stem cell , neurogenesis , genetics , inhibitory postsynaptic potential
The rostral migratory stream (RMS) is a well defined migratory pathway for precursors of olfactory bulb (OB) interneurons. Throughout the RMS an intense astroglial matrix surrounds the migratory cells. However, it is not clear to what extent the astroglial matrix participates in migration. Here, we have analyzed the migratory behavior of neuroblasts cultured on monolayers of astrocytes isolated from areas that are permissive (RMS and OB) and nonpermissive (cortex and adjacent cortical areas) to migration. Our results demonstrate robust neuroblast migration when RMS‐explants are cultured on OB or RMS‐astrocytes, in contrast to their behavior on astroglia derived from nonpermissive areas. These differences, mediated by astrocyte‐derived nonsoluble factors, are related to the overexpression of extracellular matrix and cell adhesion molecules, as revealed by real‐time qRT‐PCR. Our results show that astroglia heterogeneity could play a significant role in migration within the RMS and in cell detachment in the OB. © 2009 Wiley‐Liss, Inc.