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Tubulin polymerization‐promoting protein (TPPP/p25) is critical for oligodendrocyte differentiation
Author(s) -
Lehotzky Attila,
Lau Pierre,
Tőkési Natália,
Muja Naser,
Hudson Lynn D.,
Ovádi Judit
Publication year - 2009
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20909
Subject(s) - microbiology and biotechnology , downregulation and upregulation , biology , transfection , microtubule , oligodendrocyte , cell culture , neuroscience , biochemistry , myelin , central nervous system , genetics , gene
TPPP/p25, a recently identified tubulin polymerization‐promoting protein (TPPP), is expressed mainly in myelinating oligodendrocytes of the CNS. Here, we show that TPPP/p25 is strongly upregulated during the differentiation of primary oligodendrocyte cells as well as the CG‐4 cell line. The microRNA expression profile of CG‐4 cells before and after induction of differentiation was established and revealed differential regulation of a limited subset of microRNAs. miR‐206, a microRNA predicted to target TPPP/p25, was not detected in oligodendrocytes. Overexpression of miR‐206 led to downregulation of TPPP/p25 resulting in inhibition of differentiation. Transfection of siRNAs against TPPP/p25 also inhibited cell differentiation and promoted cell proliferation, providing evidence for an important role of TPPP/p25 during oligodendrogenesis. These results support an essential role for TPPP/p25 in oligodendrocyte differentiation likely via rearrangement of the microtubule system during the process elongation prior to the onset of myelination. © 2009 Wiley‐Liss, Inc.