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Oct4 is expressed in human gliomas and promotes colony formation in glioma cells
Author(s) -
Du Zhanhui,
Jia Deyong,
Liu Shangming,
Wang Fuwu,
Li Gang,
Zhang Yanmin,
Cao Xinmin,
Ling EngAng,
Hao Aijun
Publication year - 2009
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20800
Subject(s) - biology , glioma , nestin , stem cell , embryonic stem cell , carcinogenesis , downregulation and upregulation , cell culture , cellular differentiation , neural stem cell , microbiology and biotechnology , cancer stem cell , cancer research , cell growth , neurosphere , adult stem cell , cancer , gene , genetics
There is increasing evidence that self‐renewal capacity of cancer cells is critical for carcinogenesis; hence, it is vital to examine the expression and involvement of self‐renewal regulatory genes in these cells. Here, we reported that Oct4, a well‐known regulator of self‐renewal in embryonic stem cells, was highly expressed in human gliomas and glioma cell lines, and the expression levels were increased in parallel with increasing glioma grades. In in vitro cell cultures, Oct4 was only expressed in rat C6 glioma cells and rat neural stem cells but not in rat brain differentiated cells. Downregulation of Oct4 expression by RNA interference in C6 cells was associated with reduced cell proliferation and colony formation. Further analysis revealed that Oct4 could upregulate phosphorylation of Stat3 to promote tumor cell proliferation. Overexpression of Oct4 in C6 cells increased the expression of nestin but decreased the expression of GFAP suggesting that Oct4 might inhibit the differentiation of glioma cells. Our findings may provide further evidence for the stem cell theory of carcinogenesis. In contrast, the results might also imply that Oct4 contributes to the existence of undifferentiated cells in gliomas. © 2008 Wiley‐Liss, Inc.