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Rocks are expressed in brain tumors and are required for glioma‐cell migration on myelinated axons
Author(s) -
Oellers Patrick,
Schröer Uwe,
Senner Volker,
Paulus Werner,
Thanos Solon
Publication year - 2008
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20777
Subject(s) - rock1 , biology , glioma , laminin , rho kinase inhibitor , microbiology and biotechnology , rho associated protein kinase , extracellular matrix , cell migration , neuroscience , kinase , cancer research , in vitro , protein kinase a , biochemistry
The interactions between migrating glioma cells and myelinated fiber tracts are poorly understood. We identified that C6 glioma cells can migrate along myelinated chicken retinal axons in a novel coculture, thereby expressing small GTPases of the Rho family and serine/threonine Rho‐associated kinases (ROCKs). We found that the ROCK1 isoform is also highly expressed in native human high‐grade gliomas. Glioma cells migrated faster in vitro along myelinated axons than on laminin‐1, with the former but not the latter being specifically and reversibly blocked by the ROCK inhibitor Y27632. These data suggest that the mechanisms underlying the migration of glioma cells on myelinated axons differ from those underlying the migration on extracellular matrix molecules such as laminin‐1. © 2008 Wiley‐Liss, Inc.